Portugal winger Nani has been ruled out of the World Cup after suffering an injury to his collar bone.

Manchester United's Nani picked up the injury in training on Friday in Lisbon before flying to South Africa.

"After tests we conclude he is unfit to participate in the World Cup," said a team statement.

The 23-year-old, who scored three goals in 11 appearances during qualification, will be replaced in Portugal's squad by Benfica midfielder Ruben Amorim.

Amorim made 24 appearances for Benfica last season, helping the club to their first Portuguese title in five years.

The loss of Nani is a blow to Portugal as the winger had demonstrated impressive form for Manchester United during the second half of the 2009/10 season.